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Figure 3 | BMC Neuroscience

Figure 3

From: Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence

Figure 3

Interaction of MOR and GPR177 in PC12 cells and rat brain. (A) Lysates prepared from PC12 cells were immunoprecipitated with anti-MOR antibodies. (B) Lysates were prepared from total rat brain and immunoprecipitated with anti-MOR antibodies. In A and B, immunocomplexes were separated by SDS-PAGE, transferred to a PVDF filter, and filters probed with anti-GPR177 antibodies. Molecular weight markers (kDa) are shown at the left. The upper band in rat brain lysates is non-specific. Data shown is representative of at least three separate experiments.

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