Figure 8

LDCV secretion occurs throughout neurons. Neurons were transfected with vector encoding PAM-GFP. After 2 days in culture, cells were stimulated with PMA in the presence of PAM ectodomain antibody for 15 min; internalized antibody was subsequently visualized in fixed, permeabilized cells with a Cy3 conjugated secondary antibody. Internalized PAM antibody was detected throughout the cell soma (top), along processes (bottom) and at growth cones (labeled). In the absence of secretagogue, almost no antibody uptake was observed (not shown).