Figure 4

An intact actin cytoskeleton is required for efficient LDCV transport. Trigeminal ganglion neurons were treated with 5 μM cytochalasin B for 1 hour and then fixed and processed for immunocytochemistry (A). Filamentous actin was visualized by staining with a TRITC-phalloidin conjugate (red) and microtubules were stained with an antibody to βIII tubulin (green). Inset: phalloidin staining is shown at higher magnification to illustrate puncta that appeared in cytochalasin-treated neurons. Neurons expressing PHM-GFP were pretreated with cytochalasin B in the same manner before live imaging; cumulative velocity (B) and cumulative linearity (C) were plotted as in Figure 3.