Skip to main content
Figure 3 | BMC Neuroscience

Figure 3

From: Dynamics of peptidergic secretory granule transport are regulated by neuronal stimulation

Figure 3

LDCV dynamics in growth cones and axons are fundamentally different under basal conditions. For analysis under basal conditions, neurons were kept in phenol-free L15 medium at or above 30°C. LDCVs containing PHM-GFP were tracked in growth cones and distal portions of axons. Examples of X-Y LDCV trajectories are shown; one showed rapid, directed motion (#219), and the other moved in a slow, dithering manner (#235). Velocity data for identified LDCV trajectories were pooled from 6 cells. The distribution of binned data is shown in the histogram (B), with the cumulative plot (C) emphasizing the differences between the two regions. LDCV trajectories were also analyzed to determine the distribution of linearity (D). Linearity was calculated by comparing how close the actual path was to a straight line representing the distance between the first and last point tracked. Cumulative direction was analyzed based on the mean angle of the trajectory (E) and assigned a score of 1 if anterograde or 0 if retrograde. N = 2760 LDCVs tracked.

Back to article page