Examples of the histological evaluation of CHAT positive cells in the ventral horn of the lumbar spinal cord of neonatal mice after sciatic nerve axotomy. Sections from the lumbar spinal cord were stained with CHAT antibodies. (A, B) Examples of motoneurons in the ventral horn contralateral and ipsilateral to the lesion side in BEG -/- CaMK -/- mice (wild type littermates). (C, D) Examples of lumbar sections contra- and ipsilateral to the lesion from a BEG -/T CaMK -/- littermate (non-G-CSF expressing). (E, F) Examples of lumbar sections contra- and ipsilateral to the lesion from a G-CSF overexpressor (BEG -/T CaMK -/T mouse). (B, D, F) There are more CHAT-positive cells detectable 4 days after axotomy on the ipsilateral side in mice overexpressing G-CSF (BEG -/T CaMK -/T). All photomicrographs with 20× original magnification (OM), size bar 50 μM.