The G-CSF receptor is expressed by motoneurons in the spinal cord of wt neonatal mice. (A-C) Double fluorescence immunostaining of G-CSF receptor and CHAT (choline acetyltransferase) in the neonatal mouse spinal cord ventral horn. CHAT is used as a marker for motoneurons. All defined α motoneurons express G-CSFR (indicated by an arrow). All photomicrographs with 40× original magnification (OM), size bar 25 μm.