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Figure 7 | BMC Neuroscience

Figure 7

From: Acute NMDA toxicity in cultured rat cerebellar granule neurons is accompanied by autophagy induction and late onset autophagic cell death phenotype

Figure 7

NMDA challenge interaction with LC3-II flux and turnover. A) Lysates were obtained from control neuronal cultures (at 24 h), cultures treated with proteasome inhibitor lactasystin (20 μM) or with liposomal proteolysis inhibitor E64d (20 μM), or and 24 hours from neuronal cultures treated with NMDA (300 μM) or NMDA+lactacystin or E64d preincubation for 2 h. LC3-II immunoblot band intensities were quantified densitometrically and the values plotted. The expressed values are means ± S.E.M. [n = 3; *p < 0.05** p < 0.01 (Student paired T-test) compared to controls; § (p < 0.05] shows difference between NMDA+ lactacystin or E64d vs. lactacystin or E64d alone, respectively.

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BMC Neuroscience

ISSN: 1471-2202

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