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Figure 1 | BMC Neuroscience

Figure 1

From: Acute NMDA toxicity in cultured rat cerebellar granule neurons is accompanied by autophagy induction and late onset autophagic cell death phenotype

Figure 1

NMDA excitotoxicity results in the induction of LC3-positive autophagosomes in rat cerebellar granule neurons. Representative laser confocal fluorescent micrographs of cerebellar granule neurons in culture exposed to NMDA (200 μM) with or without co-treatment with 3-MA. NeuN (red) was used to stain mature neurons Arrowheads (yellow) represent the increased LC3 (green) staining of autophagosomes in the cell bodies of the neurons co-localized with neuronal marker NeuN (red). Red arrowheads represent the increased intensity of LC3 staining in the neuronal cell bodies at 8 hours. Neurons treated with serum free medium are labeled as controls (Ctrl) while neurons exposed to amino acid starvation conditions depict positive controls (Starv) for the induction of autophagy. All images were taken at 400× magnification. Scale bar represents 20 μm.

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BMC Neuroscience

ISSN: 1471-2202

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