Figure 1

Schematic representation of lentiviral and adeno associated constructs. All viral particles express green fluorescent protein (GFP) under the cytomegalovirus (CMV) promoter flanked by the Woodchuck hepatitis posttranscriptional regulatory element (WPRE). In the lentiviral (LV) transfer vector (A) the shRNA expression cassette, driven by the H1 RNA promoter (H1), is placed back to back with the GFP expression cassette. In the adeno associated viral vector (AAV) the shRNA expression cassette is placed upstream of the CMV promoter (B). The packaging cassettes are flanked by inverted terminal repeats (ITR) or long terminal repeats (LTR) for the AAV and LV cassettes respectively.