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Figure 4 | BMC Neuroscience

Figure 4

From: Impaired neurogenesis, learning and memory and low seizure threshold associated with loss of neural precursor cell survivin

Figure 4

Altered neurogenesis in adult SurvivinCamcremice. (A, B) Dorsal view of control (wt) and SurvivinCamcre(ko) whole brains at 16 weeks, reveals OB hypoplasia in SurvivinCamcremice. (C, D) Nissl stained sagittal sections of littermates, shows thinner cortex (double arrow) and absence of the RMS in the SurvivinCamcremice. (E, F, I, J) BrdU labeling of sagittal sections of brains from 12 week old mice shows proliferation in the SVZ-RMS pathway (E, F) and in the SGZ (I, J), that is reduced only in the SVZ of SurvivinCamcremice (F) as compared with controls (E). (G, H) DCX immunostaining of sagittal sections through the forebrain of control (G) and SurvivinCamcre(H) mice. (M, O) Quantification of BrdU+ cells in the SVZ (M) and the SGZ (O) was performed as detailed in Methods. There was a significant reduction in BrdU+ cells in the SVZ, but not in the SGZ of SurvivinCamcremice as compared to controls. (K, L) Coronal sections through the anterior SVZ of control (K) and SurvivinCamcre(L) mice were stained for tunel+ apoptotic cells (arrows). (N, P) The number of tunel+ cells in the SVZ (N) and the SGZ (P) of control and SurvivinCamcremice was quantified as described in Methods. There was a significant increase in tunel+ cells in the SVZ but not in the SGZ of SurvivinCamcremice as compared to controls. LV, lateral ventricle. Results in panels M, N, O, P are reflected as means + SEM, n = 4-5 mice per group. *P < 0.005. Scale bars: A-D 2 mm; E-H 500 μm; I-L 100 μm. (C-L) 12 weeks.

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