Figure 4

Altered neurogenesis in adult Survivin^Camcremice. (A, B) Dorsal view of control (wt) and Survivin^Camcre(ko) whole brains at 16 weeks, reveals OB hypoplasia in Survivin^Camcremice. (C, D) Nissl stained sagittal sections of littermates, shows thinner cortex (double arrow) and absence of the RMS in the Survivin^Camcremice. (E, F, I, J) BrdU labeling of sagittal sections of brains from 12 week old mice shows proliferation in the SVZ-RMS pathway (E, F) and in the SGZ (I, J), that is reduced only in the SVZ of Survivin^Camcremice (F) as compared with controls (E). (G, H) DCX immunostaining of sagittal sections through the forebrain of control (G) and Survivin^Camcre(H) mice. (M, O) Quantification of BrdU⁺ cells in the SVZ (M) and the SGZ (O) was performed as detailed in Methods. There was a significant reduction in BrdU+ cells in the SVZ, but not in the SGZ of Survivin^Camcremice as compared to controls. (K, L) Coronal sections through the anterior SVZ of control (K) and Survivin^Camcre(L) mice were stained for tunel+ apoptotic cells (arrows). (N, P) The number of tunel+ cells in the SVZ (N) and the SGZ (P) of control and Survivin^Camcremice was quantified as described in Methods. There was a significant increase in tunel+ cells in the SVZ but not in the SGZ of Survivin^Camcremice as compared to controls. LV, lateral ventricle. Results in panels M, N, O, P are reflected as means + SEM, n = 4-5 mice per group. *P < 0.005. Scale bars: A-D 2 mm; E-H 500 μm; I-L 100 μm. (C-L) 12 weeks.