The Scholl analysis technique for assessing dendritic complexity was adapted for use in brightfield Z-stack images of Golgi-Cox stained neurons. The diagram shows an illustration of Scholl lines superimposed at 20 μm increments on a Z-stack of images of a stained neuron. This was done using Zeiss AxioVision software. Each Z-stack image is separated by only 0.280 μm allowing all dendritic intersections with Scholl lines to be clearly seen and counted. Drawing not to scale.