Figure 5
From: Signaling involved in neurite outgrowth of postnatally born subventricular zone neurons in vitro
Analysis of phosphatidylinositol (PIP) involvement in the polarization of SVZ/RMS-derived neurons. Cultured cells were treated with different PIPs, and polarization of Tuj1-positive cells was determined at DIV4. (A) - (D) Examples of vehicle (A), PIP3,4,5 (B), PIP3,4 (C) and PIP4,5 (D) -treated SVZ/RMS cultures are shown. Arrowheads mark polarized neurons. (E) Treatment with PIPs significantly decreased number of polarized neurons in SVZ/RMS culture (* - p < 0.01). Abbreviations: Tuj1 - tubulin beta III to label immature neurons. Scale bars are 50 μm.