Figure 3
From: Signaling involved in neurite outgrowth of postnatally born subventricular zone neurons in vitro
Neurite outgrowth analysis of SVZ/RMS-derived neurons using genetic manipulations. Cultured cells were transfected by both pEGFP and particular gene coding construct. (A) - (H) Examples of overexpression after transfections with control (A), Pik3r1 (B), constitutively active Akt1 (C), constitutively active Cdc42 (D), inactive Cdc42 (E), Prkcz (F), constitutively active Rac1 (G) and inactive Rac1 (H) constructs are shown. (I) Overexpression of constitutively active as well as full-length gene versions increased the total neurite length while inactive gene versions decreased it (* - p < 0.005). Abbreviations: Akt1 act - constitutively active form of Akt1, C - control (pCMV-SPORT6), Cdc42 act - constitutively active form of Cdc42, Cdc42 ina - inactive form of Cdc42, Pik3r1 - full-length Pik3r1 form, Prkcz - full-length PKCζ form, Rac1 act - constitutively active form of Rac1, Rac1 ina - inactive form of Rac1, Tuj1 - tubulin beta III to label immature neurons. Scale bars are 50 μm.