Table 1 Primary antibodies used for immunohistology
From: A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons
Antigens | Antibodies | Dilutions | Companies |
|---|---|---|---|
DCX | goat anti-DCX | 1:200 | Santa Cruz, sc-8066 |
choline acetyltransferase (CHAT) | goat anti-CHAT | 1:100 | Millipore, Chemicon, AB144P |
Cre recombinase | rabbit anti-Cre | 1:500 | Abcam, ab24608 |
GFAP | rabbit anti-GFAP | 1:1000 | DAKO, Z0334 |
GAD65 | rabbit anti-GAD65 | 1:1000 | Millipore, Chemicon, AB5082 |
tyrosine hydroxylase (TH) | rabbit anti-TH | 1:5000 | Pel-Freez, P40101 |
calretinin | rabbit anti-calretinin | 1:2000 | Swant, 7699/4 |
ionized calcium-binding adaptor molecule 1 (Iba1) | rabbit anti-Iba1 | 1:500 | Wako, 019-19741 |
GFP | chicken anti-GFP | 1:1000 | Aves, GFP1020 |
beta-galactosidase | chicken anti-beta-galactosidase | 1:1000 | Abcam, ab9361 |
NeuN | mouse anti-NeuN | 1:200 | Millipore, Chemicon, MAB377 |
calbindin D-28K | mouse anti-calbindin-D-28K | 1:2000 | Sigma-Aldrich, C9848 |
2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) | mouse anti-CNPase, | 1:200 | Millipore, Chemicon, MAB326R |
NG2 chondroitin sulfate proteoglycan | mouse anti-NG2 | 1:500 | Millipore, Upstate, 05-710 |
5-bromodeoxyurudine (BrdU) | rat anti-BrdU | 1:500 | AbD Serotec, OBT0030CX |
vesicular glutamate transporter 2 (VGLUT2) | guinea pig anti-VGLUT2 | 1:5000 | Millipore, Chemicon, AB5907 |