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Table 1 Primary antibodies used for immunohistology

From: A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons

Antigens Antibodies Dilutions Companies
DCX goat anti-DCX 1:200 Santa Cruz,
sc-8066
choline acetyltransferase (CHAT) goat anti-CHAT 1:100 Millipore, Chemicon, AB144P
Cre recombinase rabbit anti-Cre 1:500 Abcam, ab24608
GFAP rabbit anti-GFAP 1:1000 DAKO,
Z0334
GAD65 rabbit anti-GAD65 1:1000 Millipore, Chemicon, AB5082
tyrosine hydroxylase (TH) rabbit anti-TH 1:5000 Pel-Freez, P40101
calretinin rabbit anti-calretinin 1:2000 Swant,
7699/4
ionized calcium-binding adaptor molecule 1 (Iba1) rabbit anti-Iba1 1:500 Wako,
019-19741
GFP chicken anti-GFP 1:1000 Aves,
GFP1020
beta-galactosidase chicken anti-beta-galactosidase 1:1000 Abcam,
ab9361
NeuN mouse anti-NeuN 1:200 Millipore, Chemicon, MAB377
calbindin D-28K mouse anti-calbindin-D-28K 1:2000 Sigma-Aldrich, C9848
2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) mouse anti-CNPase, 1:200 Millipore, Chemicon, MAB326R
NG2 chondroitin sulfate proteoglycan mouse anti-NG2 1:500 Millipore, Upstate,
05-710
5-bromodeoxyurudine (BrdU) rat anti-BrdU 1:500 AbD Serotec, OBT0030CX
vesicular glutamate transporter 2 (VGLUT2) guinea pig anti-VGLUT2 1:5000 Millipore, Chemicon, AB5907