Figure 3

Co-localization of EGFP and DCX expression following TAM administration. Immunodetection of DCX (red) and EGFP (green) in the neurogenic regions of adult DCX-CreERT2:CAG-CAT-EGFP mice 2 days (a-d), 8 days (e-h) and 15 days (i-l) following last TAM administration. The extensive co-localization of DCX and EGFP observed at D2 rapidly decrease over time. The frequency of co-localization between EGFP and DCX was quantified at various time points. Percentages of EGFP-labeled cells among all DCX-expressing cells decreased over time in the SVZ (m) and in the DG (n). The efficiency of recombination, i.e. the percentages of all DCX+ cells that expressed EGFP, in shown in (o), whereas the specificity, i.e. the percentages of all EGFP+ cells expressing DCX is shown in (p). LV: lateral ventricle. Scale bar in (c) = 33 μm