Induction of reporter gene expression by TAM injections at different developmental stages. a) Immunodetection of DCX expression in an E14.5 mouse embryo. Detection of β-gal activity at E15.5 in a DCX-CreERT2: Rosa26lacZ embryos injected with (b) TAM or (c) vehicle at E14.5. Arrow in (b) points to a labeled dorsal root ganglion. No β-gal signal could be detected in vehicle-injected embryos. Immunodetection of DCX (red), NeuN (blue) and EGFP (green) in the DG (d and e) and SVZ (f and g) of a 2 month-old DCX-CreERT2:CAG-CAT-EGFP mouse injected with TAM at E17.5. Arrowheads in (f) point to EGFP-expressing cells remaining at the ependymal lining. Immunodetection of DCX (red), NeuN (blue) and EGFP (green) in the adult OB (h and i) and DG (j and k) of DCX-CreERT2:CAG-CAT-EGFP mice 4 weeks after TAM injection in the adulthood. Arrow in (i) points at the co-labeling EGFP with DCX at the anterior end of the RMS. Scale bars = 1.7 mm in (a), 2 mm (b) and (c), 50 μm (k).