Figure 5

Effects of NDNF on cultured hippocampal neurons. A. The cultured neurons were transfected with pEGFP to express green fluorescent protein (GFP) and immunostained with the neuron marker, neuN antibody (red). B. The conditioned medium with NDNF protein was used on cultured hippocampal neurons, with BDNF (20 ng/ml) as positive controls, and quantitative analysis was provided in C-E. C. A histogram was used to graphically display the number of cells that migrated per field (n = 10). BDNF or NDNF vs control, p < 0.01. D. A histogram was used to graphically display the cell soma area (n = 60). BDNF or NDNF vs control, p < 0.05. E. A histogram was used to graphically display the neurite length (n = 60). BDNF or NDNF vs control, NDNF vs BDNF, p < 0.01. Scale bars: 50 um.