Glycosylation, and tissue distribution of NDNF. A. Mouse NDNF protein immunoprecipitated from brain extracts was incubated with PNGase F and then analyzed by western blot in triplicate. B. Protein samples from the brains, spinal cords, kidneys, livers and hearts of mice were analyzed by western blot analysis using beta-actin as a control in triplicate. NDNF is expressed by cells in the mouse brain and spinal cord.