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Table 2 Ca2+ permeability of coexpressed mutant NMDAR subunits: Reversal potentials and PCa2+/Pmono ratios

From: Residues at the tip of the pore loop of NR3B-containing NMDA receptors determine Ca2+ permeability and Mg2+block

  Erev(Gly)[mV] (n) PCa2+/Pmono± SEM (Gly) (n) Erev(Glu/Gly)[mV] (n) PCa2+/Pmono± SEM (Glu/Gly) (n)
NR1-3a/NR2B -23.1 ± 3.93 (5) 3.1 ± 0.65 (5) -29.3 ± 1.79 (5) 2.3 ± 0.21 (5)
NR1-3a/NR2B(GN) -15.3 ± 5.06 (5) 4.5 ± 1.02 (5) -32.3 ± 2.73 (5) 2.0 ± 0.27 (5)
NR1-3a(GS)/NR2B -39.7 ± 2.05 (5) 1.4 ± 0.13 (5) -44.7 ± 2.20 (5) 1.1 ± 0.10 (5)
NR1-3a(GS)/NR2B(GN) -46.8 ± 5.14 (5) 1.0 ± 0.18 (5) -44.1 ± 1.24 (5) 1.1 ± 0.07 (5)
NR1-3a/NR3B -68.4 ± 5.65 (9) 0.4 ± 0.11 (9) -64.9 ± 4.26 (8) 0.5 ± 0.11 (8)
NR1-3a/NR3B(NR) -34.7 ± 5.27 (9) 1.7 ± 0.61 (9) -28.2 ± 1.71 (8) 2.5 ± 0.21 (8)
NR1-3a/NR3B(GN) -49.2 ± 7.66 (9) 0.4 ± 0.31 (9) -36.3 ± 2.75 (8) 1.7 ± 0.21 (8)
NR1-3a/NR3B(NS) -46.8 ± 10.03 (5) 1.0 ± 0.57 (5) -20.4 ± 4.74 (4) 2.5 ± 0.79 (4)
NR1-3a(GS)/NR3B -64.5 ± 12.62 (5) 0.2 ± 0.20 (5) -76.2 ± 7.92 (5) 0.1 ± 0.07 (5)
NR1-3a(GS)/NR3B(NR) -83.1 ± 14.72 (5) 0.1 ± 0.13 (5) -93.2 ± 10.33 (5) 0.1 ± 0.04 (5)
NR1-3a(GS)/NR3B(GN) -54.6 ± 7.02 (5) 0.3 ± 0.11 (5) -59.5 ± 7.61 (5) 0.3 ± 0.07 (5)
NR1-3a(GS)/NR3B(NS) -38.0 ± 1.16 (5) 0.7 ± 0.03 (5) -38.2 ± 2.74 (5) 0.7 ± 0.10 (5)
  1. Overview of reversal potentials (Erevs) and calculated PCa2+/Pmono ratios of diheteromers featuring N and N+1 site mutant NMDAR subunits measured upon activation by either glycine alone (Gly, 10 μM) or Gly in addition to glutamate (Glu, 100 μM). Reversal potentials were measured in 8 mM Ca2+ Ringer. Errors represent the standard error of the mean (SEM). n = number of cells.
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