Effect of Hypoxic stress on regulation of PRDX6 and NF-κB proteins in RGCs exposed to 1% O
. Cells (4 × 105) were cultured in 100 mm culture plates, and after 24 h these cells were exposed to 1% O2 for a period of 24 and 48 h. After treatment, cell extracts were prepared for Western analysis. A significant increase in PRDX6 protein expression was observed after 24 h and reduced after 48 h of 1% O2 exposure (lane 2). In contrast, an increase in NF-κB protein level was observed (middle panel) while no change was detected in the expression of β-actin level (lower panel), suggesting hypoxia specifically reduced the expression of PRDX6. Histogram shows relative density (Pixels) of protein bands. *p < 0.05.