Western analysis showing modulated expression of PRDX6 and NF-κB protein in RGCs treated with CoCl
. Cells (4 × 105) were cultured in 100 mm culture plates, and after 24 h these cells were treated with different concentrations of CoCl2 (50, 100, 200 μM) for a period of 24, 48 or 72 h. After treatment, cell extracts were prepared for Western analysis. A significant reduction of PRDX6 protein expression was observed following treatment with 100 or 200 μM of CoCl2 at 72 h (upper panel, PRDX6; 72 h; lanes 2, 3 and 4, 48 h; lanes 3 and 4). In contrast, an increase in NF-κB protein level was observed (middle panel) while no change was detected in the expression of β-actin level (lower panel), suggesting hypoxia specifically reduced the expression of PRDX6. Histogram shows relative density (Pixels) of protein bands. *p < 0.05.