Altered distribution of flotillin-1 in neuronal DRMs isolated with different detergents. A. Equal volumes of primary cortical lysates were incubated with the indicated detergents at a final concentration of 1% (w/v) prior to fractionation by sucrose gradient centrifugation. Equal volumes of each fraction was analysed by Western blotting with an antibody to flotillin-1. Apparent molecular mass is indicated to the left. All blots are representative of at least three independent experiments. B. Quantification of flotillin-1 in neuronal DRM fractions isolated with indicated detergents expressed as % DRM enrichment of total gradient flotillin-1; n = 3.