Representative photomicrographs of immunostaining after transient brainstem ischemia for 15 min (cochlear nucleus). Left column shows immunoreactivity for MAP2 (A-E); middle column shows immunoreactivity for IBA-1 (F-J); right column shows immunoreactivity for GFAP (K-O). Ischemic lesions with loss of immunoreactivity for MAP2 were seen in the ventral cochlear nucleus (VC) at 3 days and 7 days after reperfusion (D and E; red arrowheads). Clusters of IBA-1-positive amoeboid microglia/macrophages (I and J; red arrowheads) and loss of GFAP expression (N and O; red arrowheads) were detected in the same areas where MAP2 expression was lost at 3 days and 7 days after reperfusion. Increased immunoreactivity for GFAP (N and O; red arrowheads) was also detected around ischemic lesions at 3 days and 7 days after reperfusion. Scale bars = 0.5 mm.