Figure 4From: Delayed neuronal cell death in brainstem after transient brainstem ischemia in gerbilsRepresentative photomicrographs of immunostaining after transient brainstem ischemia for 15 min (cochlear nucleus). Left column shows immunoreactivity for MAP2 (A-E); middle column shows immunoreactivity for IBA-1 (F-J); right column shows immunoreactivity for GFAP (K-O). Ischemic lesions with loss of immunoreactivity for MAP2 were seen in the ventral cochlear nucleus (VC) at 3 days and 7 days after reperfusion (D and E; red arrowheads). Clusters of IBA-1-positive amoeboid microglia/macrophages (I and J; red arrowheads) and loss of GFAP expression (N and O; red arrowheads) were detected in the same areas where MAP2 expression was lost at 3 days and 7 days after reperfusion. Increased immunoreactivity for GFAP (N and O; red arrowheads) was also detected around ischemic lesions at 3 days and 7 days after reperfusion. Scale bars = 0.5 mm.Back to article page