Presence of cAPPL in central nervous system and other tissues. a) Relative levels of mRNA in different tissues. b) Western blot of protein levels in different tissues using an antibody against APP (upper) and actin (lower). c) Western blot of the pre-adsorption assay. Fresh Control, antibody non-incubated; 48 h Control, antibody incubated without peptide; Pre-adsorbed, antibody incubated with the peptide. d) Western blot of the immunoprecipitation using an antibody against APP. Mock control corresponding to immunoprecipitation with preimmune rabbit serum. The arrowhead indicates the 55kDa band corresponding to the heavy chain of the antibody.