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Table 1 c-Fos counts in reward-related regions following an injection of either vehicle or picrotoxin into the SuM or its vicinity

From: Administration of the GABAA receptor antagonist picrotoxin into rat supramammillary nucleus induces c-Fos in reward-related brain structures. Supramammillary picrotoxin and c-Fos expression

    

c-Fos counts in a 325 × 425 μm area

 

Region

  

PIC-SUM

(N = 14)

PIC-OUT

(N = 7)

VEH

(N = 9)

  

Distance (mm)

Hemi-sphere

Mean

P-value

Mean

P-value

Mean

Cortex

Medial prefrontal cortex

2.8

i

71.4

0.0042

40.7

0.2695

26.3

  

2.8

c

60.2

0.0399

30.6

0.8129

27.7

 

Retrosplenial granular cortex

-2.8

i

50.2

0.0572

45.4

0.0402

5.7

  

-2.8

c

35.8

 

32.7

 

8.4

 

Hippocampus dentate gyrus

-2.8

i

44.1

 

14.4

 

1.6

  

-2.8

c

41.3

 

7.9

 

1.9

 

Basolateral nucleus of amygdala

-2.8

i

11.8

 

18.3

 

3.3

  

-2.8

c

12.9

 

17.3

 

4.6

Basal forebrain

Central nucleus of amygdala

-2.8

i

6.6

 

33.1

 

8.2

  

-2.8

c

6.9

 

7.0

 

6.2

 

Lateral septal nucleus, dorsal part

2.0

i

43.7

0.0565

56.3

0.0263

11.4

  

2.0

c

38.7

 

41.1

 

12.2

  

1.0

i

13.0

 

24.1

 

1.6

  

1.0

c

10.3

 

22.3

 

1.6

  

0.0

i

18.4

0.0261

6.4

0.4434

1.8

  

0.0

c

17.4

0.0369

10.6

0.1427

1.3

 

Lateral septal nucleus, intermediate part

2.0

i

72.9

0.0009

16.6

0.5070

5.8

  

2.0

c

43.9

0.0016

13.4

0.5993

8.7

  

1.0

i

194.1

0.0004

74.4

0.1148

7.7

  

1.0

c

99.9

0.0045

35.4

0.3642

12.4

  

0.0

i

57.1

0.0084

26.1

0.1900

4.3

  

0.0

c

45.3

0.0086

17.0

0.3078

3.7

 

Lateral septal nucleus, ventral part

2.0

i

150.1

0.0009

76.9

0.0730

18.0

  

2.0

c

86.3

0.0084

57.0

0.0887

22.2

  

1.0

i

297.4

0.0002

102.9

0.1898

32.3

  

1.0

c

158.1

0.0017

52.6

0.6105

36.6

  

0.0

i

139.6

0.0031

96.9

0.0205

8.6

  

0.0

c

81.1

0.0065

61.4

0.0202

6.9

 

Septohypothalamic nucleus

0.0

i

127.1

0.0009

55.6

0.1279

12.0

  

0.0

c

73.0

0.0144

54.9

0.0423

11.7

 

Medial septal nucleus

1.0

i

17.2

 

10.4

 

6.4

  

0.0

c

18.9

0.0136

14.7

0.0290

1.7

 

Nucleus of the vertical limb of the diagonal band

1.0

i

21.9

0.0356

10.0

0.2844

1.6

  

1.0

c

6.2

 

10.1

 

1.1

 

Nucleus of the horizontal limb of the diagonal band

1.0

i

19.0

0.0097

11.6

0.0778

1.6

  

1.0

c

4.3

 

6.1

 

1.7

 

Nucleus accumbens core

2.0

i

31.4

 

4.6

 

2.9

  

2.0

c

25.9

0.0434

2.0

0.8836

3.6

  

1.0

i

12.6

 

3.4

 

2.1

  

1.0

c

9.6

 

3.4

 

2.8

 

Nucleus accumbens shell, medial part

2.0

i

48.2

0.0012

7.1

0.8499

5.1

  

2.0

c

43.8

0.0022

10.6

0.6573

6.1

  

1.0

i

23.4

0.0202

12.9

0.1577

2.4

  

1.0

c

18.6

0.0361

8.4

0.3962

3.4

 

Olfactory tubercle

2.0

i

7.4

 

5.9

 

1.3

  

2.0

c

6.4

 

12.0

 

1.9

Thalamus

Paraventricular thalamic nucleus

-1.0

i

50.2

 

29.4

 

39.2

  

-1.0

c

41.4

 

29.9

 

36.0

  

-2.0

m

72.1

 

79.4

 

76.1

 

Central medial thalamic n

-2.0

m

43.6

 

45.4

 

31.1

 

Lateral habenula

-3.0

i

18.7

 

18.9

 

18.2

  

-3.0

c

47.1

0.0494

24.0

0.4813

14.7

Hypothalamus

Medial preoptic area

0.0

i

34.1

 

15.7

 

9.8

  

0.0

c

18.6

 

12.1

 

11.3

 

Lateral preoptic area

0.0

i

45.5

0.0313

50.3

0.0422

7.3

  

0.0

c

30.0

 

37.6

 

8.1

  

-1.0

i

35.3

0.0118

39.9

0.0120

2.2

  

-1.0

c

19.7

0.0083

35.9

0.0001

2.4

 

Dorsomedial hypothalamic nucleus

-3.0

i

47.7

0.0042

6.9

0.6072

12.7

  

-3.0

c

36.6

0.0826

7.4

0.4753

15.8

 

Lateral hypothalamic area

-2.0

i

30.1

0.0120

22.0

0.0444

4.9

  

-2.0

c

20.0

0.0166

23.1

0.0131

4.3

 

Supramammillary n

-4.0

m

187.0

0.0021

141.0

0.0170

48.3

Midbrain

Anterolateral VTA

-5.0

i

23.1

0.0603

4.9

0.7089

1.4

  

-5.0

c

1.8

 

1.9

 

1.6

 

Posterior VTA

-5.8

i

9.6

0.0135

4.1

0.2728

1.0

  

-5.8

c

3.7

 

3.6

 

1.0

 

Central linear n

-6.7

m

3.9

 

3.6

 

1.0

 

Dorsal raphe n

-7.8

m

21.1

0.0251

16.0

0.0498

1.7

 

Median raphe n

-7.8

m

15.4

 

20.1

 

1.3

  1. Notes: c-Fos counts in bold indicate significant differences from respective counts for vehicle injections. Blank space in the p-value columns indicates that the effect of region was not significant, following an ANOVA/MANOVA test. Abbreviations: c, contralateral to injection site; i, ipsilateral to injection site; m, midline; n, nucleus