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Figure 7 | BMC Neuroscience

Figure 7

From: Diacylglycerol kinase β promotes dendritic outgrowth and spine maturation in developing hippocampal neurons

Figure 7

Overexpression of DGKβ in hippocampal neurons at 13 DIV. Cultured neurons were transfected at 13 DIV with GFP-DGKβ (A), GFP-DGKβKD (B), and control vector (GFP) (C). After 24 h incubation, they were fixed and stained with Vybrant-DiI (red) to outline the shape of dendritic spines. Note that mature spines of mushroom-type (A, arrowheads) are frequently observed in neurons transfected with GFP-DGKβ, but hardly with GFP-DGKβKD (B, arrows) and control vector (C, arrows). (D) Histogram showing the density of mature spines per 500 μm dendrite in neurons transfected with GFP-DGKβ (n = 5 neurons), GFP-DGKβKD (n = 7 neurons), and control vector (n = 6 neurons). The data are the mean ± S.E.M. *P < 0.05; NS, statistically not significant. Scale bars, 5 μm.

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