Figure 5

Overexpression of DGKβ in hippocampal neurons at 6 DIV. Cultured neurons were transfected at 6 DIV with GFP-fused wild-type DGKβ (GFP-DGKβ) (A), kinase-dead mutant of DGKβ (GFP-DGKβKD) (B), and control vector (GFP) (C). After 24 h incubation, they were fixed and stained with phalloidin to label F-actin (red). In each confocal image (A-C), boxed area is enlarged below. GFP-DGKβ is mostly observed along dendrites as puncta, which largely colocalize with clustered F-actin (arrowheads). On the other hand, GFP-DGKβKD and GFP show a diffuse pattern of localization (arrows). Scale bars, 25 μm (upper column in A-C); 5 μm (lower column in A-C).