Figure 2

Double immunofluorescence for characterization of DGKβ-expressing neurons in the hippocampus. In all images, DGKβ is colored in red. Green fluorescence represents glutamate decarboxylase (A-D, GAD), microtubule associated protein-2 (E, MAP2), vesicular γ-aminobutylic acid transporter (F, VGAT), or postsynaptic density 95 kDa (G, PSD-95). Blue fluorescence represents vesicular glutamate transporter 1 (F, VGluT1). (A-D) DGKβ is co-expressed not only in scattered GAD-positive interneurons (*), but also in widely distributed GAD-negative projection neurons in the CA1, CA2, and dentate gyrus subfields. DGKβ is also detected in a GAD-positive non-pyramidal cell in the stratum oriens (A, arrowheads). (E) DGKβ is distributed on the surface of MAP-2-positive dendrites. (F and G) Punctate immunolabeling of DGKβ is not overlapped with that of presynaptic terminal markers, VGAT and VGluT1 (F), but partially with that of postsynaptic terminal marker, PSD-95 (G). Or, stratum oriens; Py, pyramidal layer; DG, dentate gyrus; Gr, granule cell layer. Scale bars, 50 μm (A); 10 μm (B-E); 1 μm (F); 4 μm (G).