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Figure 4 | BMC Neuroscience

Figure 4

From: Integrating microRNA and mRNA expression profiles of neuronal progenitors to identify regulatory networks underlying the onset of cortical neurogenesis

Figure 4

Differentially expressed mRNAs detected by microarrays were validated with qRT-PCR and immunohistochemistry. A. The cycle threshold of Klf7 is shifted to the left in E13 ENPs confirming an increase in Klf7 mRNA expression level, while the beta-actin mRNA expression level remains nearly constant. B. The fold changes from E11 to E13 for seven transcription factors identified by microarrays (black bars) and qRT-PCR (grey bars) are plotted. C, D. Cutl1 protein (red fluorescence) is expressed at E13 in the differentiating zone (DZ) of the ventral (C) and dorsal (D) telencephalon. The blue fluorescence depicts nuclear counterstain with DAPI. The lateral ventricle (LV) is at bottom of the figure. NE = neuroepithelium. Scale bar = 20 microns.

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BMC Neuroscience

ISSN: 1471-2202

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