Figure 6

Morphology of neurons within neural aggregate and MNP cultures. Distribution of βtubulin⁺ neurons within secondary neural aggregates (a; b, i + iii) and MNP cultures (b, ii + iv). Two weeks after the initiation of differentiation, neural aggregate-derived βtubulin⁺ neurons are located within secondary aggregates (a overview: i, stacked set of optical slice at the edge indicated by white box in i: ii) and on top of flat-elongated interjacent cells (b, i + iii). Higher magnifications of neural aggregate- (b, iii) and MNP- (b, iv) derived βtubulin⁺ neurons reveal differences in neurite length and morphology. Scale bar = 100 μm (a, i; b, i + ii); 20 μm (a, i; b, iii + iv).