Figure 3
In vivo analysis of CpG methylation within the c-mycERTAM promoter region in CTX0E03 cells. CpG dinucleotide-containing regions (underlined) examined in CMV promoter of the c-mycERTAM transgene (A). Methylation sequence analysis of the CMV transgene promoter region of in vivo and in vitro samples (B). Ten animal grafted brains were analyzed, five at 1-week (top set) and five at 4-weeks (bottom set), in addition, a non-implanted CTX0E03 cell sample (control). Ten clones containing the sequence depicted in (A) from each grafted rat brain were analyzed. Each row shown represents a clone. Filled circles = methylated CpG island; open circles = unmethylated CpG island. Percentages of global methylation are reported at the bottom of each sample.