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Table 2 The effect of adaptor protein signaling at Y490 TrkB for Kv1.3 current properties.

From: Brain-derived neurotrophic factor modulation of Kv1.3 channel is disregulated by adaptor proteins Grb10 and nShc

Transfection Condition Peak Current
(pA)
τInact
(msec)
τDeact
(msec)
Kv1.3 + TrkB    
   Pre 356 ± 71 (5) 697 ± 130 32.2 ± 2.1
   Post *298 ± 64 780 ± 59 24.2 ± 2.9
Kv1.3 + TrkBShc-    
   Pre 372 ± 144 (3) 693 ± 45 31.8 ± 3.3
   Post 386 ± 140 843 ± 114 26.5 ± 3.7
Kv1.3 + TrkBShc- + nShc    
   Pre 533 ± 109 (10) 1070 ± 163 31.2 ± 4.3
   Post 473 ± 115 1040 ± 196 35.3 ± 3.8
Kv1.3 + TrkBShc- + Grb10    
   Pre 533 ± 122 (4) 894 ± 173 29.0 ± 1.6
   Post *379 ± 84 660 ± 229 32.6 ± 7.8
  1. HEK 293 cels were transfected with cDNAs encoding Kv1.3 +TrkB kinase or TrkBShc- (Y490 mutation) ± adaptor protein. Several voltage-stimulating paradigms were completed (see "Methods") to collect the tabled biophysical properties by cell-attached patch-clamp recording. BDNF stimulation, notations, and statistical analysis as in Table 1.