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Table 1 The effect of adaptor proteins on TrkB kinase-induced Kv1.3 current properties.

From: Brain-derived neurotrophic factor modulation of Kv1.3 channel is disregulated by adaptor proteins Grb10 and nShc

Transfection Condition Modulator Peak current
(pA)
τInact
(msec)
τDeact
(msec)
V1/2
(mV)
κ
Kv1.3 BDNF      
   Pre   287 ± 49 (4) 794 ± 252 31.5 ± 5.4 -38.2 ± 5.8 4.2 ± 0.7
   Post   303 ± 68 961 ± 263 38.3 ± 13.2 -41.5 ± 6.8 4.9 ± 1.1
Kv1.3 + Grb10 BDNF      
   Pre   506 ± 271 (4) 774 ± 110 31.3 ± 4.5 -39.4 ± 6.1 5.4 ± 0.4
   Post   498 ± 246 *610 ± 127 34.2 ± 4.7 -41.5 ± 6.8 4.4 ± 2.6
Kv1.3 + nShc BDNF      
   Pre   543 ± 176 (7) 770 ± 45 32.6 ± 4.3 -45.0 ± 2.5 4.3 ± 0.6
   Post   586 ± 107 817 ± 54 31.4 ± 4.0 -41.4 ± 3.3 3.9 ± 0.9
Kv1.3 + TrkB Vehicle      
   Pre   551 ± 198 (4) 788 ± 186 37.2 ± 3.3 -40.4 ± 5.0 5.1 ± 0.5
   Post   557 ± 183 742 ± 130 32.3 ± 3.3 -38.6 ± 5.8 5.4 ± 0.7
Kv1.3 + TrkB BDNF      
   Pre   555 ± 173 (7) 900 ± 243 36.8 ± 2.9 -36.2 ± 4.6 5.8 ± 1.1
   Post   *442 ± 173 762 ± 151 38.8 ± 6.7 -40.3 ± 6.7 5.3 ± 1.4
Kv1.3 + TrkB + Grb10 BDNF      
   Pre   488 ± 114 (7) 862 ± 110 29.3 ± 3.5 -42.6 ± 3.7 6.7 ± 0.6
   Post   545 ± 133 *553 ± 46 35.4 ± 5.5 *-54.1 ± 2.8 6.3 ± 1.4
Kv1.3 + TrkB + nShc BDNF      
   Pre   343 ± 71 (13) 618 ± 67 37.4 ± 3.2 -40.9 ± 1.9 4.4 ± 0.7
   Post   381 ± 92 549 ± 68 36.4 ± 2.0 -43.5 ± 2.9 4.4 ± 1.1
  1. HEK 293 cells were transfected with cDNAs encoding Kv1.3 ± TrkB ki nase ± adaptor protein. Several voltage-stimulating paradigms were completed (see "Methods") to collect the tabled biophysical properties by cell-attached patch-clamp recording. Mean values (± s.e.m.) for peak current amplitude (pA), inactivation time constant (τInact), deactivation time constant (τDeact), voltage at half activation (V1/2), and slope (κ) of the voltage dependence were compared across various transfection conditions at the start of the patch recording (Pre) versus twenty minutes following 1 ng/μg BDNF (Post) using a paired t-test, α ≤ 0.05. * = Significantly different.