Figure 2

Co-localization of nShc and Kv1.3 in the olfactory bulb of postnatal mice. Ten micron coronal cryosections were sequentially, double immunolabeled with α AU13 (Kv1.3 antiserum, 1:500) and αShc (1:500), and visualized by incubation in species-specific fluorophore-conjugated secondary antibodies where the green channel represents Kv1.3 channel, the red channel represents Shc adaptor, and the merged image (co-localization of proteins) is yellow. Note that the channel and adaptor overlap in the peripheral structures surrounding the glomeruli and in the external plexiform layer (EPL), the mitral cell layer (MCL), the internal plexiform layer (IPL), and the granule cell layer (GCL). ONL = outer nerve layer.