Figure 4

Western blot analyses of p-ERK, p-CREB and p-ERα protein levels and the effects of Ct and (or) SU on them in cerebral ischemia. Cantharidin (Ct) and (or) SU were administered at 24 h reperfusion after ischemia. (A) Effect of SU and co-administration with Ct and SU on p-ERK in plasma membrane and cytoplasm (M and C) following post-ischemic reperfusion, (B) Effect of SU and co-administration with Ct and SU on p – ERK in nucleus (N), (C) Effect of SU and co-administration with Ct and SU on p – CREB following post-ischemic reperfusion, (D) Effect of SU and co-administration with Ct on p – ERα following post-ischemic reperfusion. The results are expressed as fold changes versus the sham controls. O.D. is presented as fold – increase compared to Veh controls. Data are expressed as mean ± S.D. (n = 4/group), ^#P < 0.05 vs. the respective SU administration group.