Table 2 PCR primers used in this study
From: Tissue-specific and neural activity-regulated expression of human BDNF gene in BAC transgenic mice
Primer/application | Sequence |
|---|---|
BAC modification | |
hBDNFcod_rpsL_neo_s | 5' GGATAGACACTTCTTGTGTATGTACATTGACCATTAAA AGGGGAAGATAGGGCCTGGTGATGATGGCGGGATCG 3' |
hBDNF_rpsL_neo_as | 5'AATAGATAATTTTTGTCTCAATATAATCTAATCTATACAACATAAATCCATCAGAAGAACTCGTCAAGAAGG 3' |
hBDNFcod_linker_EGFP_s | 5' TAAGGATAGACACTTCTTGTGTATGTACATTGACCAT TAAAAGGGGAAGACGGGATCCACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTG 3' |
hBDNF_EGFP_as | 5' AATAGATAATTTTTGTCTCAATATAATCTAATCTATAC AACATAAATCCATTACTTGTACAGCTCGTCCATGCCGA 3' |
genotyping/slot-blot hybridization/expression analysis | |
hBDNF_s | GTACGTGCGGGCCCTTACCATGGATAGC |
EGFP_as | TGGTGCAGATGAACTTCAGGGTCAGC |
expression analysis | |
mBDNF_s | GTATGTTCGGGCCCTTACTATGGATAGC |
mBDNF_as | AAGTTGTGCGCAAATGACTGTTTC |
HPRT1_s | CTTTGCTGACCTGCTGGATTAC |
HPRT1_as | GTCCTTTTCACCAGCAAGCTTG |
hBDNF_I_s | GATGCCAGTTGCTTTGTCTTCTGTAG |
hBDNF_II_s | GGGCGATAGGAGTCCATTCAGCACC |
hBDNF_III_s | AGTTTCGGGCGCTGGCTTAGAG |
hBDNF_IV_s | GCTGCAGAACAGAAGGAGTACA |
hBDNF_V_s | TCGCGTTCGCAAGCTCCGTAGTG |
hBDNF_Vh_s | GGCTGGAACACCCCTCGAA |
hBDNF_VI_s | GGCTTTAATGAGACACCCACCGC |
hBDNF_VII_s | GAACTGAAAGGGTCTGCGACACTCT |
hBDNF_IXb_s | GCTGCTAAAGTGGGAAGAAGG |
hBDNF_IX_as1 | GTCCTCATCCAACAGCTCTTCTATC |
hBDNF_IX_as2 (with VII_s) | GAAGTGTACAAGTCCGCGTCCTTA |
expression analyis (qPCR) | |
EGFPq_s | CAGAAGAACGGCATCAAGGTG |
EGFPq_as | TGGGTGCTCAGGTAGTGGTTG |
hBDNFq_I_s | CAGCATCTGTTGGGGAGACGAGA |
hBDNFq_IV_s | GAAGTCTTTCCCGGAGCAGCT |
hBDNFq_VI_s | ATCGGAACCACGATGTGACT |
hBDNFq_IXc_s | AACCTTGACCCTGCAGAATGGCCT |
hBDNFq_IX_as1 (with I, IV_s) | ATGGGGGCAGCCTTCATGCA |
hBDNFq_IX_as2 (with VI_s) | ACCTTGTCCTCGGATGTTTG |
hBDNFq_IX_as3 (with IXc_s) | GATGGTCATCACTCTTCTCACCT |
mBDNFq_I_s | TTGAAGCTTTGCGGATATTGCG |
mBDNFq_IV_s | GAAATATATAGTAAGAGTCTAGAACCTTG |
mBDNFq_VI_s | GCTTTGTGTGGACCCTGAGTTC |
mBDNFq_IXa_s | GGACTATGCTGCTGACTTGAAAGGA |
mBDNFq_IX_as1 (with I, IV, VIs) | AAGTTGCCTTGTCCGTGGAC |
mBDNFq_IX_as2 (with IXa_s) | GAGTAAACGGTTTCTAAGCAAGTG |
mBDNFq_coding_s | GGCCCAACGAAGAAAACCAT |
mBDNFq_coding_s | AGCATCACCCGGGAAGTGT |
HPRT1q_s | CAGTCCCAGCGTCGTGATTA |
HPRT1q_as | AGCAAGTCTTTCAGTCCTGTC |
transgene integrity | |
pBACe3.6_SP6 (5'end) | TATTTAGGTGACACTATAG |
rp11_5'_as (5'end) | GGACAACAGACCCAAGGAGA |
rp11_3'_s (3'end) | GTAGGGTGTCTGGGTTGGTG |
pBACe3.6_T7 (3'end) | TAATACGACTCACTATAGGG |
transgene tandem integration | |
rp11_3'_s (P1) | GTAGGGTGTCTGGGTTGGTG |
pBACe_11326_s (P2) | CGGTTACGGTTGAGTAATAAATGGATG |
pBACe_11365_s (P3) | GGGGCACATTTCATTACCTCTTTCTC |