Figure 3
From: Role of serotonergic neurons in the Drosophila larval response to light
Larvae expressing active TNT in Ddc neurons present increased response to light throughout development. Photobehavioral responses measured as Response index (RI) of Ddc: TNT larvae tested in the ON/OFF assay at different developmental stages. RI = [(Distance traveled (pixels) in dark (OFF) pulses - distance traveled (pixels) in light (ON) pulses)/Total distance traveled (pixels) during the assay]. In agreement with the results obtain using DIAS, analyses of the RI values obtained using the semi-automatic system show that, compared to control Ddc:TNT-VIF larvae, Ddc:TNT-G larvae displayed increased response to light throughout larval development (late 2nd instar (46–49 h AH): UAS-TNT-G/+;Ddc-GAL4/+, n = 11, RI = 0.52; UAS-TNT-VIF/+;Ddc-GAL4/+, n = 12, RI = 0.29; ANOVA: F(1,21) = 21.53, p < 0.001; early foraging 3rd instar (65–68 h AH): UAS-TNT-G/+;Ddc-GAL4/+, n = 35, RI = 0.50; UAS-TNT-VIF/+;Ddc-GAL4/+, n = 23, RI = 0.28; ANOVA: F(1,56) = 62.79, p < 0.001; late foraging 3rd instar (72–75 h AH): UAS-TNT-G/+;Ddc-GAL4/+, n = 15, RI = 0.51; UAS-TNT-VIF/+;Ddc-GAL4/+, n = 14, RI = 0.28; ANOVA: F(1,27) = 30.92, p < 0.001; early wandering 3rd instar (91–94 h AH):UAS-TNT-G/+;Ddc-GAL4/+, n = 24, RI = 0.31; UAS-TNT-VIF/+;Ddc-GAL4/+, n = 23, RI = 0.14; ANOVA: F(1,45) = 23.38, p < 0.001; late wandering 3rd instar (96–99 h AH):UAS-TNT-G/+;Ddc-GAL4/+, n = 27, RI = 0.33; UAS-TNT-VIF/+;Ddc-GAL4/+, n = 24, RI = 0.13; ANOVA: F(1,49) = 43.36, p < 0.001). *** p < 0.001.