Figure 7

NET colocalizes extensively with syntaxin 1A but less so with SCAMP2. SCGs were double-labeled with NET-05 and with antibodies directed against syntaxin 1A or SCAMP2. The first column (A, and E) represents the DIC images of an exemplary field of cells whereas the second column (B, F, green) is the distribution of the NET in the same or fibers or boutons. Arrow in A highlights bouton shown in inset. The third column (C, G, red) shows images obtained with antibodies against syntaxin 1A or SCAMP2. The last column (D, H) represents the merge of signals obtained with NET-05 and the appropriate synaptic membrane proteins. Insets in staining panels provide a higher power view of single boutons and are highlighted in full images with arrows. I. Correlation of NET and SCAMP2 pixel densities (I, upper left), ICA analysis (lower panels), and spatial display of PDM values (I, upper right). J. Correlation of NET and syntaxin 1A pixel densities (upper left), ICA analysis (lower panels) and spatial display of PDM values (J, upper right). PDM color transformation is shown above standard red/green visualizations of same field.