Figure 4

Visualization of NET expression in cultured SCGs. Cultured mouse SCGs were immunolabeled with monoclonal NET-05 (permeabilized) or NET 43408 (nonpermeabilized) antibodies. Shown are single confocal sections and corresponding DIC images from SCG cultures stained with NET-05 antibody and obtained from WT (A-I) or NET KO (J-L) animals. NET-05 recognizes NET in both cell bodies (B) and in axons (E), as noted by arrows. No signal was detected in either cell bodies or axons in the NET KO neurons (H, K). Scale bar = 10 μM. M-O: Staining of SCG cultures under nonpermeabilized conditions with ectodomain NET antibody 43408. Note selective localization of NET staining to the surface of SCG boutons (arrows). Scale bar = 10 μM.