NET-05 monoclonal antibody specifically recognizes rodent NET. A. Alignment of the NET-05 peptide antigen with mNET amino terminus (starting at the first amino acid) and related monoamine transporters from various species. B. Representative Western blot analysis of cell extracts from nontransfected COS-7 cells or from COS-7 cells expressing either rat or human NET (rNET and hNET, respectively). C. Western blot assessment of NET expression in mouse brain (S1 fraction, cortex) using S1 membranes from either wild type or NET KO mice.