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Figure 6 | BMC Neuroscience

Figure 6

From: Kalirin12 interacts with dynamin

Figure 6

Kalirin12 inhibits transferrin endocytosis in cultured cortical neurons. A. Dissociated cortical neurons were nucleofected with vectors encoding GFP (upper) or myc-Kalirin12 (bottom) and examined after 6 days. AlexaFluor546-transferrin (red) was internalized for 5 min, cells were rinsed and fixed for visualization of Kalirin12 (myc; green) or GFP and βIII tubulin (blue). White arrows identify the cell soma. Scale bar, 10 μm. Including an acidic wash to remove surface transferrin did not alter the results. B. Transferrin signal in the cell soma was quantified in ≥ 20 images from GFP and Kalirin12 expressing neurons. Soma area and total red intensity were quantified. SEM is indicated; bracket, p < 0.001 using two-tailed student T-test.

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