Sequence conservation of PMP22 in jawed vertebrates. (A) Phylogenic tree of amino acid sequences of the EMP/PMP22 family in jawed vertebrates was drawn by using clustelX and NJ plot. The numbers at the nodes are bootstrap values based on 10,000 replications. Sequences from each subfamily clustered together. The red arrowhead shows the corresponding sequence of ol_pmp22. (B) The amino acid alignment consisting of the mammalian, avian, amphibian, fish pmp22 genes and medaka fish paralogs was drawn by using clustelX and GeneDoc. The white letter on the black background indicates 100 percent conserved blocks. The white letter on the dark gray background indicates 80 percent or greater conserved blocks. The black letter on the bright gray background indicates 60 percent or greater conserved blocks. Red and blue squares indicate conservation of the N41 glycosylation site (NXS/T) and C terminus ER-retention/retrieval signal (LRKRE), respectively. Hs: Homo sapiens (human), Mm: Mus musculus (mouse), Rn: Rattus norvegicus (rat), Gg: Gallus gallus (chicken), Xt: Xenopus tropicalis, Xl: Xenopus laevis, Tn: Tetraodon nigroviridis, Tr: Takifugu rubripes, Ga: Gasterosteus aculeatus (stickleback), Ol: Oryzias latipes (medaka fish), Dr: Danio rerio (zebrafish).