Table 3 Sequences of primers used for real-time semiquantitative PCR reactions
Primer | Acc. N° | Sequence (5'-3') | Amplified product (bp) |
|---|---|---|---|
Bax | AF235993 | 5'-tgctacagggtttcatccag-3' | 135 |
5'-ccagttcatcgccaattcg-3' | |||
Bcl-2 | NM_031535 | 5'-tggaaagcgtagacaaggagatgc-3' | 88 |
5'-caaggctctaggtggtcattcagg-3' | |||
c-jun | X17163 | 5'- aacgaccttctacgacgatg-3' | 140 |
5'-ggtgcggcttcagattgc-3' | |||
NGF | M36589 | 5'- acctcttcggacactctgg-3' | 165 |
5'- cgtggctgtggtcttatctc-3' | |||
P75 | NM_012610 | 5'- agtggcatctctgtggac-3' | 130 |
5'- ctacctcctcacgcttgg-3' | |||
TrkA | NM_021589 | 5'- aagccgtggaacagcatc-3' | 91 |
5'- agcacagagccgttgaag-3' | |||
VEGF | AY702972 | 5'-accctggctttactgctgtac-3' | 103 |
5'- cgtccatgaacttcaccacttc-3' | |||
FLT-1 | NM_019306 | 5'- tgtcctcaactgcaccgtcac-3' | 100 |
5'- ccgctgcctgatagatgctctc-3' | |||
FLK-1 | NM_013062 | 5'- tggcaaatacaacccttcag-3' | 108 |
5'- cacactcagtcaccaacac-3' | |||
GAPDH | M17701 | 5'-ggcaagttcaatggcacagtcaag-3' | 125 |
5'-acatactcagcaccagcatcacc-3' |