Real time PCR of Bax, Bcl-2 and c-jun genes. The induction of hypoperfusion induces an increase in the pro-apoptotic gene Bax mRNA level (2VO-saline versus sham-saline) in the retina (B) which then decreases after NGF treatment. The relative expression of the anti-apoptotic gene Bcl-2 conversely increases in the 2VO-NGF group compared with the sham-saline group of animals (A). The vascular lesion also causes the induction of c-jun mRNA expression (2VO-saline versus sham-saline) (D), which is counteracted by NGF intravitreal injection (E). Experiments were performed in triplicate. Results are presented as mean values ± SEM. Statistical analyses performed: one-way ANOVA with multiple comparison post hoc Tukey's test (#p < 0.05) and Student's t test (*p < 0.05; **p < 0.01).