A, B: Histological staining (Hematoxylin-Eosin) of the retina of sham-operated (A) and 2VO ligated 8 days after surgery (B). C, D: Histological staining (Masson Trichromic staining) of the optic nerve of sham (C) and 2VO animals 8 days after surgery (D). E-R: Optic nerve staining of sham- (E-L) and 2VO- operated animals 8 days after surgery (M-R) with the markers listed in the figure. Images are obtained by conventional fluorescence microscopy. Bars: 100 μm (A, B); 200 μm (C-R). Abbreviations: ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer;GCL: ganglion cell layer.