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Figure 6 | BMC Neuroscience

Figure 6

From: Statistical evaluation of methods for quantifying gene expression by autoradiography in histological sections

Figure 6

Transformation of grey levels. Grey levels from 0 to 255 were transformed using three different methods (A). The uncalibrated relative OD and the Rodbard OD method are plotted on the left y-axis, while transformation with the 14C-standard uses the right y-axis. The box indicates the range of observed grey level values in this dataset and are re-plotted in (B). All three methods give different curves, however within the range of actual values, the transformation is fairly linear, especially for the relative and Rodbard OD transforms.

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