Expression analysis of Neil3 in embryonic brain development. (A) Real-time quantitative PCR was performed on mRNA isolated from mouse brains at four different developmental stages. The data were normalized to Gapdh and expressed relative to the level of Neil3 at P0. (B) A detailed expression profile of Neil3 during brain development was obtained by using a mouse developmental tissue qPCR array. The array contains one concentration of cDNAs, normalized to Gapdh. Neil3 mRNA levels were calculated relative to the sample expressing the lowest level of Neil3 (Fron Cx, P7). The experiment was conducted twice and the standard deviations are indicated. Abbreviations: Tel, telencephalon; Die, diencephalon; Mes, mesencephalon (midbrain); Rhom, Rhombencephalon; Sp C, spinal cord; Mid B, midbrain; Med, medulla; Fron Cx, frontal cortex; Post Cx, posterior cortex; Ent Cx, entorhinal cortex; Olf B, olfactory bulb; Hipp, hippocampus; Str, Striatum; Tha, thalamus; Hyp, hypothalamus; Cer, cerebellum.