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Figure 4 | BMC Neuroscience

Figure 4

From: Cellular and subcellular localization of Marlin-1 in the brain

Figure 4

Localization of Marlin-1 relative to synapses. (A-C) Cultured hippocampal neurons were fixed at 14 div and co-labeled with antibodies to Marlin-1 (green) and Piccolo (PICC, red). (D-F) High magnification of the area boxed in C. Scale bars, 10 μm (A-C). Arrows, neurite axis; arrowheads, synapses. (G-J) Pre-embedding immunogold EM of the CA1 region of the hippocampus incubated with Marlin-1 antibodies. Marlin-1 is found in the cytoplasm of dendritic shafts (Den, arrows), and occasionally in dendritic spines (s, crossed arrow) but not in presynaptic boutons (b). Scale bar, 0.5 μm (G-J).

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