Results from the data analysis of donepezil treated APP/PS1 (cortex/hippocampus). (A) PLS-DA scatter plot which displays a clear separation between mice before and after donepezil treatment (white circles = before treatment and black circles after treatment). Each data point represents one mouse. A Q2-value above 0.05 indicates that the groups can be significantly distinguished along that component. (B) Metabolites which are important for the separation between groups in the PLS-DA model. VIP (variable of importance in the projection) values larger than 1 are relevant in explaining group differences (black bars) while metabolites with values below 1 have no significant impact (white bars). (C) The paired t-test shows a significant decrease in Tau, p = 0.019. White diamonds represent before treatment and black squares after treatment.