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Figure 2 | BMC Neuroscience

Figure 2

From: Effects of NR1 splicing on NR1/NR3B-type excitatory glycine receptors

Figure 2

Comparison of glutamate/glycine-induced steady-state current amplitudes for all NR1 isoforms with either NR2B or NR3B. A. Relative current responses for each of the eight NR1 splice variants coexpressed with NR2B (upper panel) or NR3B (lower panel). Amplitudes were normalized to the responses mediated by NR1-1a/NR2B and NR1-1a/NR3B, respectively. All currents were recorded after the application of 300 μM glutamate and 10 μM glycine. Data shown here are mean values ± Gaussian error propagation, n = 6–10 from 2 independent experiments per combination. B. Current potentiation by the N-terminal NR1b splice variants over NR1a variants when coexpressed with either NR2B (light grey) or NR3B (dark grey). Responses of the NR1a variants were independently set to 1 and responses mediated by NR1b variants were normalized to the responses of the respective NR1a variants. Data are mean values ± Gaussian error propagation, n = 6–10 from 2 independent experiments per combination. ns = not significant. C. Relative glutamate/glycine-induced current responses of NR1–3a- and NR1–3b-containing NMDARs depending on the pH value. Current responses were normalized to the values measured at pH 7.2. Data are mean values ± Gaussian error propagation, n = 6. alone = expressed alone; ns = not significant.

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